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THE EFFECT OF CHEMICAL SYMPATHECTOMY ON MITOCHONDRIAL FUNCTION IN THE ISCHAEMIC AND REPERFUSED MYOCARDIUM
Author(s) -
NAYLER WINIFRED G.,
SCOTT ELIZABETH M.
Publication year - 1982
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1982.tb09350.x
Subject(s) - ischemia , sympathectomy , saline , mitochondrion , medicine , anesthesia , chemistry , cardiology , endocrinology , biochemistry
1 Isolated rabbit hearts were perfused aerobically for 120 min, made ischaemic for 90 min, or made ischaemic for 90 min and then reperfused for 30 min. 2 Some rabbits were pretreated with 6‐hydroxydopamine (6‐OHDA), given as three separate intravenous doses of 30, 20 and 20 mg/kg, 20 to 48 h before they were killed; others (controls) received saline according to the same regime. 3 Mitochondria were harvested from left ventricular homogenates and their function assessed by measuring state 3 O 2 consumption (state 3 Q O 2 ), respiratory control index (RCI), phosphate: oxygen ratio (ADP:O), Ca 2+ content, and ATP‐producing activity. In other experiments peak left ventricular developed tension was recorded. 4 In hearts from saline‐treated animals, mitochondrial state 3 Q O 2 , RCI and ATP producing activities were reduced after global ischaemia, with or without reperfusion. There was a small gain in mitochondrial Ca 2+ after ischaemia, and a large gain upon reperfusion. 5 6‐OHDA pretreatment provided some protection against the effects of ischaemia and reperfusion on mitochondrial function and on peak developed tension. 6 It was concluded that chemical sympathectomy with 6‐OHDA does not duplicate the effect of prolonged β‐adrenoceptor blockade in protecting mitochondrial function against the deleterious effects of ischaemia and reperfusion.