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RENAL VASODILATOR ACTIVITY OF PROSTAGLANDIN E 2 IN THE RAT ANAESTHETIZED WITH PENTOBARBITONE
Author(s) -
HAYLOR J.,
TOWERS J.
Publication year - 1982
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1982.tb09198.x
Subject(s) - vasodilation , pentobarbital , prostaglandin , prostaglandin e , pharmacology , vasodilator agents , chemistry , medicine , anesthesia , endocrinology
1 The effect of intra‐aortic administration (i.a.) of prostaglandin E 2 (PGE 2 ) on renal blood flow was studied in the rat anaesthetized with pentobarbitone. Renal blood flow was assessed in two ways, either by use of an electromagnetic flow probe or by measurement of the renal clearance of p ‐aminohippurate (PAH). 2 PGE 2 (0.1 μg/min, i.a.) increased renal blood flow measured by either method. However, PAH clearance overestimated the degree of vasodilatation compared to that obtained using the flow meter. The possibility that PGE 2 or a metabolite may increase PAH extraction by the kidney was considered. 3 The sensitivity of the rat to the renal vasodilator actions of PGE 2 was enhanced by using a flank retro‐peritoneal approach from which to insert the flow probe, rather than a mid‐line abdominal incision. 4 Dose‐response curves demonstrate that under the conditions used, PGE 2 produced a biphasic change in renal vascular resistance, vasodilatation started at 0.01 μg/min and was maximal at about 3 μg/min, while at the highest dose used (20μg/min) PGE 2 induced renal vasoconstriction. 5 The results indicate that contrary to previous reports, the rat does not exhibit an important species difference in the response of its renal vasculature to PGE 2 . Therefore, physiological and pathophysiological roles which have previously been attributed to vasoconstriction produced by PGE 2 synthesized in the kidney may now have to be considered.