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STIMULATION OF SODIUM AND OF CHLORIDE TRANSPORT IN EPITHELIA BY FORSKOLIN
Author(s) -
CUTHBERT A.W.,
SPAYNE J.A.
Publication year - 1982
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1982.tb09187.x
Subject(s) - forskolin , stimulation , sodium , chemistry , biophysics , chloride , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , organic chemistry
The diterpene, forskolin, is shown to produce a concentration‐dependent, increase in short circuit current in two epithelial preparations, amphibian skin and rat colon. In the amphibian tissue the increase is sensitive to amiloride and due to an increase in electrogenic transepithelial sodium transport towards the serosal side. In the rat colon piretanide attenuated the forskolin effect, suggesting the terpene increases electrogenic transepithelial chloride transport towards the mucosal side. Half‐maximal activation of both processes was achieved with concentrations of 1–3 μ m , similar to those required to activate half‐maximally the catalytic subunit of adenylate cyclase.