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LOW CONCENTRATIONS OF PROSTAGLANDIN E 2 INHIBIT THE PROSTACYCLIN‐INDUCED ELEVATION OF CYCLIC ADENOSINE 3′,5′‐MONOPHOSPHATE IN ELICITED POPULATIONS OF RAT PERITONEAL MACROPHAGES
Author(s) -
ADOLFS MARTIN J.P.,
BONTA IVAN L.
Publication year - 1982
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1982.tb08796.x
Subject(s) - prostacyclin , adenosine , endocrinology , medicine , prostaglandin e2 , prostaglandin , cyclic adenosine monophosphate , cyclic nucleotide , in vitro , prostaglandin e , chemistry , adenosine monophosphate , biology , biochemistry , nucleotide , receptor , gene
1 Elevation of cyclic adenosine 3′,5′‐monophosphate (cyclic AMP) in elicited populations of rat peritoneal macrophages was used as a parameter to examine the influence of prostaglandin E 2 (PGE 2 ) on the effects of prostacyclin (PGI 2 ) and (±)‐5E‐13,14‐didehydro‐carbo‐prostacyclin (DDH‐carbo‐PGI 2 ) in vitro . 2 PGE 2 , within the range of 1.4 × 10 −9 to 1.2 × 10 −8 m , caused a concentration‐dependent inhibition of the rise in cyclic AMP induced by 2.8 × 10 −6 m PGI 2 or DDH‐carbo‐PGI 2 . 3 With higher concentrations of PGE 2 the inhibition was either non‐existent or masked by the effect of PGE 2 per se on cyclic AMP levels. 4 The present findings suggest that the earlier observed low responsiveness of granuloma macrophages to PGI 2 , in terms of rise in cyclic AMP, is possibly due to permanent exposure of these cells to environmental endogenous PGE 2 .