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ANOMALOUS COMPARTMENTATION OF 5‐HYDROXYTRYPTAMINE IN INTACT HUMAN PLATELETS
Author(s) -
COSTA J.L.,
KIRK K.L.,
MURPHY D.L.,
STARK H.
Publication year - 1981
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1981.tb10996.x
Subject(s) - compartment (ship) , extracellular , biophysics , cellular compartment , vesicle , microbiology and biotechnology , biology , chemistry , intracellular , biochemistry , cell , membrane , oceanography , geology
1 The movement of radiolabelled 5‐hydroxytryptamine (5‐HT) between the extracellular medium, the thrombin‐releasable (vesicular) compartment, and a non‐thrombin‐releasable compartment has been investigated in washed human platelets. 2 Appreciable amounts of extracellular 5‐HT can accumulate in a non‐releasable compartment. Depending on the incubation conditions and the amount of 5‐HT already present inside the cell, non‐releasable 5‐HT can either remain non‐releasable or rapidly migrate into the vesicular compartment. 3 Measurable amounts of vesicular 5‐HT can also enter a non‐releasable compartment. However, vesicular 5‐HT which becomes non‐releasable does not appear to mix with 5‐HT becoming non‐releasable following uptake from the extracellular medium. 4 Extracellular 5‐HT can be added to vesicles and to the non‐releasable compartment in the presence of appreciable quantities of 5‐HT already in one or both compartments. 5 The vesicular and non‐releasable compartments appear to accumulate 5‐HT independently of one another. Furthermore, most vesicular accumulation of 5‐HT occurs from the extracellular medium, rather than by translocation of non‐releasable 5‐HT into the vesicular compartment.