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FURTHER STUDIES ON 5‐HYDROXYTRYPTAMINE TRANSPORT IN PANCREATIC ISLETS AND ISOLATED β‐CELLS
Author(s) -
LINDSTRÖM PER
Publication year - 1981
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1981.tb10433.x
Subject(s) - pancreatic islets , medicine , endocrinology , reserpine , islet , efflux , stimulation , intracellular , insulin , chemistry , biology , biochemistry
1 The transport mechanism for 3 H‐labelled 5‐hydroxytryptamine (5‐HT) in isolated pancreatic islets of non‐inbred ob/ob mice was further characterized. 2 Isolated β‐cells accumulated 5‐HT to the same degree and with the same Na + ‐dependence as whole islets. 3 Imipramine inhibited the uptake in a concentration‐dependent way. 4 Reserpine did not affect the uptake or efflux rates. 5 Glucose stimulation of insulin secretion did not affect the uptake rate. 6 It is concluded that the observed islet uptake of [ 3 H]‐5‐HT represents an intracellular accumulation by the β‐cells. Mechanisms at the level of the plasma membrane may be rate‐limiting for this process.