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THE EFFECTS OF MORPHINE AND METHIONINE‐ENKEPHALIN ON THE RELEASE OF PURINES FROM CEREBRAL CORTEX SLICES OF RATS AND MICE
Author(s) -
STONE T.W.
Publication year - 1981
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1981.tb09970.x
Subject(s) - morphine , ouabain , chemistry , purine metabolism , enkephalin , (+) naloxone , adenosine , endocrinology , purine , medicine , cerebral cortex , stimulation , pharmacology , opioid , receptor , biochemistry , sodium , enzyme , organic chemistry
1 Slices of cerebral cortex from Wistar rats, TO mice or C57 mice were preincubated with [ 3 H]‐adenosine, and labelled purines were subsequently released by electrical stimulation or by perfusing with ouabain, 100 μM 2 Electrically‐evoked purine release was substantially reduced when the Ca 2+ concentration in the medium was lowered from 2.4 to 0.1 mM. In both rats and mice, the electrically‐evoked release was increased by morphine and methionine‐enkephalin (Met‐enkephalin), 10 μm and in rats and TO mice by morphine 1 μm, both drug effects being prevented by naloxone 3 Purine release evoked by ouabain was also increased by morphine 1 and 10 μm, though not by Met‐enkephalin, from slices of rat cortex. Ouabain‐induced release from TO mice was reduced by morphine, and from C57 mice was unchanged 4 The enhancement by morphine of electrically‐evoked purine release may indicate that purines mediate some effects of morphine in the CNS.