Premium
ARACHIDONIC ACID METABOLISM AND MODULATION OF in vitro ANAPHYLAXIS BY 5,8,11,14‐EICOSATETRAYNOIC ACID AND 9a,12a‐OCTADECADIYNOIC ACID
Author(s) -
HITCHCOCK MARGARET,
KOKOLIS NICHOLAS A.
Publication year - 1981
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1981.tb09150.x
Subject(s) - arachidonic acid , guinea pig , lipoxygenase , histamine , contraction (grammar) , metabolism , anaphylaxis , chemistry , linoleic acid , pharmacology , in vitro , biochemistry , biology , medicine , endocrinology , immunology , allergy , enzyme , fatty acid
1 5,8,11,14‐Eicosatetraynoic acid (ETYA) inhibited the antigen‐induced contractions of tracheal spirals obtained from actively sensitized guinea‐pigs. Consistent data were obtained only when the spirals were treated with indomethacin. 2 ETYA did not affect histamine‐induced contractions of indomethacin‐treated tracheal spirals. 3 9a, 12a‐Octadecadiynoic acid (Ro‐3–1314) a potential inhibitor of linoleic acid metabolism, stimulated the antigen‐induced contraction of guinea‐pig tracheal spirals and the immunological release of slow reacting substance of anaphylaxis (SRS‐A) from actively sensitized guinea‐pig lung fragments. 4 Both ETYA and Ro‐3–1314 inhibited the immunological release of malondialdehyde from guinea‐pig lung fragments. 5 The data indicate that the effects of ETYA were due to inhibition of lipoxygenase and the effects of Ro‐3–1314 were due to inhibition of cyclo‐oxygenase. 6 The results suggest that products of lipoxygenase contribute to the antigen‐induced contraction of guinea‐pig lung, particularly when cyclo‐oxygenase is inhibited. Under these conditions there may be redirection of the metabolism of arachidonic acid to favour production of constrictor products of lipoxygenase such as SRS‐A.