Premium
PHAGOCYTOSIS STIMULATES THE RELEASE OF A SLOW REACTING SUBSTANCE IN CULTURED MACROPHAGES
Author(s) -
BRETZ U.,
DEWALD B.,
PAYNE T.,
SCHNYDER J.
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10983.x
Subject(s) - mepyramine , zymosan , tachyphylaxis , chemistry , substance p , arachidonic acid , phagocytosis , nordihydroguaiaretic acid , lipoxygenase , cyclooxygenase , macrophage , pharmacology , biochemistry , biology , immunology , neuropeptide , in vitro , receptor , antagonist , enzyme
1 A slow‐reacting substance (SRS) was released from non‐elicited mouse peritoneal macrophages during phagocytosis of zymosan particles, whereas no detectable SRS was produced by resting cells. 2 The macrophage SRS induced a delayed and slow contraction of the guinea‐pig ileum but not of the chick rectum. 3 The myotonic activity was antagonized by low concentrations of FPL 55712 (sodium 7‐[3‐(4‐acetyl‐3‐hydroxy‐2‐propylphenoxy)‐2‐hydroxypropoxy]‐4‐oxo‐8‐propyl‐4H‐1‐benzopyran‐2 carboxylate) but was not affected by mepyramine or hyoscine, and was not associated with tachyphylaxis. 4 SRS release was increased by indomethacin and was abolished by the lipoxygenase and cyclo‐oxygenase inhibitor, BW755C (3‐amino‐1‐[ m ‐(trifluoromethyl)‐phenyl]‐2‐pyrazoline). 5 Addition of exogenous arachidonic acid or cysteine enhanced SRS production.