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CALCIUM IONOPHORE A23187 INDUCES RELEASE OF CHEMOKINETIC AND AGGREGATING FACTORS FROM POLYMORPHO‐NUCLEAR LEUCOCYTES
Author(s) -
BRAY M.A.,
FORDHUTCHINSON A.W.,
SHIPLEY M.E.,
SMITH M.J.H.
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10964.x
Subject(s) - nordihydroguaiaretic acid , ionophore , chemokinesis , calcium , arachidonic acid , biochemistry , lipoxygenase , chemistry , metabolism , biological activity , chemotaxis , endocrinology , biology , enzyme , in vitro , receptor , organic chemistry
1 Rat and human polymorphonuclear leucocytes (PMNs) when exposed to calcium ionophore A23187 10 μ m release products which cause aggregation of rat PMNs and chemokinesis of human PMNs. 2 Aggregating and chemokinetic activities are rapidly generated; maximal release occurs after 4 min, and can be detected in dilutions of the supernatant of up to 1:1000. 3 Generation of aggregating and chemokinetic activities is inhibited by nordihydroguaiaretic acid 10 −4 to 10 −7 m , 5,8,11,14‐eicosatetraynoic acid 10 −4 and 10 −5 m , BW 755C 10 −4 m and benoxaprofen 10 −4 m , all compounds known to inhibit lipoxygenase pathways of arachidonic acid (AA) metabolism. 4 Conventional non‐steroidal anti‐inflammatory agents, such as aspirin and indomethacin, inhibited little or not at all the generation of these activities. 5 We conclude that the aggregating and chemokinetic activities induced by A23187 represent generation of biologically active products of lipoxygenase pathways of AA metabolism.