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INTERACTION BETWEEN ADENOSINE TRIPHOSPHATE AND NORADRENALINE IN THE ISOLATED VAS DEFERENS OF THE GUINEA‐PIG
Author(s) -
KAŽIĆ TOMISLAV,
MILOSAVLJEVIĆ DRAGICA
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10913.x
Subject(s) - vas deferens , phentolamine , adenosine triphosphate , medicine , adenosine , purinergic receptor , endocrinology , guinea pig , acetylcholine , tachyphylaxis , chemistry , stimulation , muscle contraction , contraction (grammar) , potassium , biology , organic chemistry
1 An interaction between adenosine triphosphate (ATP) and noradrenaline (NA) was investigated in the isolated vas deferens of the guinea‐pig. 2 ATP (1.7 to 50 μ m ) enhanced contractions due to transmural electrical stimulation (ES; parameters: 30 Hz, 0.2 to 0.3 ms, 10 to 30 V; 1 s train duration). Responses to exogenous NA (12 μ m ) were also potentiated by ATP while contractions to acetylcholine (6 μ m ) were inhibited. 3 NA (1.2 μ m ) potentiated ATP‐induced contractions and prevented the development of tachyphylaxis to ATP (510 μ m ). 4 Phentolamine (12.5 μ m ) prevented the potentiation by NA of ATP‐induced contractions; these contractions were insensitive to phentolamine (up to 25 μ m ). 5 Removal of potassium chloride from the Tyrode solution for 10 min abolished the potentiating actions of both ATP and NA. 6 The present results suggest that the effect of ATP may be functionally closely related to that of NA at α‐adrenoceptors in the vas deferens of the guinea‐pig.