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MODULATION BY ACETYLCHOLINE OF THE ELECTRICALLY‐EVOKED RELEASE OF [ 3 H]‐ACETYLCHOLINE FROM THE ILEUM OF THE GUINEA‐PIG
Author(s) -
FOSBRAEY P.,
JOHNSON E.S.
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10894.x
Subject(s) - acetylcholine , atropine , muscarinic acetylcholine receptor , myenteric plexus , ileum , cholinergic , choline , chemistry , endocrinology , stimulation , guinea pig , medicine , biology , receptor , immunohistochemistry
1 Acetylcholine (ACh) stores within neurones of the myenteric plexus of the guinea‐pig were labelled with [ 3 H]‐choline and the influence of unlabelled ACh, atropine, or atropine and unlabelled ACh on the electrically‐evoked output of [ 3 H]‐ACh was evaluated. 2 Electrical transmural stimulation (5 Hz) of the ileum led to an increase in the output of [ 3 H]‐ACh over that released spontaneously. Superfusion with unlabelled ACh (6.8 μ m ) caused a marked reduction in the release of [ 3 H]‐ACh which was reversed by atropine (3.5 μ m ). Atropine itself had no effect on the electrically‐evoked [ 3 H]‐ACh. 3 These experiments provide further evidence for the existence in the guinea‐pig ileum of neuronal muscarinic receptors for ACh subserving an inhibitory role on transmitter release.