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CHARACTERISTICS OF 5‐HYDROXYTRYPTAMINE TRANSPORT IN PANCREATIC ISLETS
Author(s) -
LINDSTRÖM PER,
SEHLIN JANOVE,
TÄLJEDAL INGEBERT
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10871.x
Subject(s) - pancreatic islets , antimycin a , endocrinology , medicine , islet , chemistry , platelet , 2,4 dinitrophenol , serotonin , kinetics , biophysics , insulin , biology , biochemistry , receptor , electron transport chain , physics , quantum mechanics
1 Transmembrane transport of 3 H‐labelled 5‐hydroxytryptamine (5‐HT) by isolated pancreatic islets of non‐inbred ob/ob mice was studied. 2 5‐HT was vigorously accumulated in a temperature‐dependent way by the islet cells. 3 Studies of the concentration‐dependence of [ 3 H]‐5‐HT uptake revealed complex kinetics with one component being saturated at 1 to 3 u m 5‐HT (apparent association constant 0.6 × 10 6 m −1 ) and the other non‐saturated up to 1 m m 5‐HT. 4 The saturable uptake was inhibited by Na + ‐deficiency and metabolic poisoning with 2,4‐dinitro‐phenol and antimycin A, whereas the non‐saturable component was not affected. 5 Omission of K + , Ca 2+ or Mg 2+ did not affect the uptake rate. 6 It is concluded that 5‐HT is taken up by pancreatic β‐cells by mechanisms very similar to those observed in thrombocytes and neurones.