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FAILURE OF ANTI‐INFLAMMATORY STEROIDS TO INHIBIT PROSTAGLANDIN PRODUCTION BY RAT POLYMORPHONUCLEAR LEUCOCYTES
Author(s) -
DRAY F.,
McCALL ELAINE,
YOULTEN L.J.F.
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb10408.x
Subject(s) - prostaglandin , prostaglandin e2 , incubation , endocrinology , prostaglandin e , medicine , dexamethasone , chemistry , glycogen , biology , biochemistry
1 Like rabbit polymorphonuclear (PMN) leucocytes, rat peritoneal glycogen‐induced PMN leucocytes produced much greater amounts of prostaglandin when incubated with killed bacteria than in the absence of phagocytosable material. 2 Rat PMN leucocytes produced mainly prostaglandin E2 (PGE2), in amounts up to 17 ng/10 6 cells in 90 min incubation, some 25 times the amount produced by resting cells. 3 Indomethacin and meclofenamic acid inhibited prostaglandin production by resting and phagocytosing cells, the IC 50 being of the order of 10 −6 to 10 −7 M for both drugs. 4 Hydrocortisone and dexamethasone at concentrations up to 10 −4 M did not cause significant dose‐related inhibition of prostaglandin production in this system. 5 It is suggested that the phagocytosing PMN leucocyte is insensitive to the action of anti‐inflammatory steroids with respect to prostaglandin production.