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ELECTRICALLY‐EVOKED RELEASE OF [ 3 H]‐HISTAMINE FROM THE GUINEA‐PIG HYPOTHALAMUS
Author(s) -
BIGGS MARILYN J.,
JOHNSON E.S.
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb09774.x
Subject(s) - guinea pig , histamine , hypothalamus , chemistry , endocrinology , medicine , biology
1 [ 3 H]‐histamine was taken up by slices of guinea‐pig hypothalamus against a concentration gradient. 2 Electrical field stimulation of the superfused slices resulted in an increased efflux of radioactivity, the major part of which was shown to be associated with histamine by paper chromatography. 3 The evoked release of histamine was dependent on calcium ions in the superfusate and was increased by 56% when the frequency of stimulation was doubled from 5 to 10 Hz.