Premium
LOCALIZATION OF SENSITIVE SITES TO TAURINE, γ‐AMINOBUTYRIC ACID, GLYCINE AND β‐ALANINE IN THE MOLECULAR LAYER OF GUINEA‐PIG CEREBELLAR SLICES
Author(s) -
OKAMOTO KOICHI,
SAKAI YUTAKA
Publication year - 1980
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1980.tb07029.x
Subject(s) - taurine , strychnine , glycine , alanine , guinea pig , purkinje cell , picrotoxin , cerebellum , inhibitory postsynaptic potential , biochemistry , amino acid , biophysics , chemistry , hepatic stellate cell , biology , neuroscience , endocrinology , gabaa receptor , receptor
1 The localization, in the molecular layer of guinea‐pig cerebellar slices, of the sites most sensitive to iontophoretically applied taurine, γ‐aminobutyric acid (GABA), glycine and β‐alanine was investigated. 2 The most sensitive sites were located (expressed as the distance from the Purkinje cell body to the pial surface): 0 μm, 60 μm and 220 μm for taurine; 0 μm and 180 μm for GABA; 80 μm and 200 μm for glycine, and 80 μm, 180 μm and 300 μm for β‐alanine. 3 The sensitive site (at 0 μm) to GABA was considered to represent the basket synapses on the Purkinje cell soma, while the sites (60 to 80 μm and 200 to 300 μm) for taurine, glycine and β‐alanine were tentatively assigned to the synapses of the stellate neurones on the Purkinje cell dendrites. 4 Inhibitory actions of all four amino acids tested at the most sensitive sites were antagonized by both picrotoxin and strychnine. 5 The possibility that taurine might be the neurotransmitter of the stellate neurones in guinea‐pig cerebellum is discussed.