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EFFECTS OF PROSTAGLANDINS E 2 AND I 2 ON HUMAN LYMPHOCYTE TRANSFORMATION IN THE PRESENCE AND ABSENCE OF INHIBITORS OF PROSTAGLANDIN BIOSYNTHESIS
Author(s) -
GORDON D.,
HENDERSON D.C.,
WESTWICK J.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb16101.x
Subject(s) - biosynthesis , transformation (genetics) , prostaglandin e , prostaglandin , dinoprostone , lymphocyte , mixed lymphocyte reaction , chemistry , endocrinology , immunology , biology , biochemistry , t cell , immune system , enzyme , gene
1 The reactivity of human peripheral blood mononuclear cells to phytohaemagglutinin ([ 3 H]‐thymidine incorporation) was enhanced in indomethacin‐ and eicosatetraynoic acid‐treated cells compared with untreated cells, from normal volunteers. This suggests that endogenously synthesized prostaglandins have an inhibitory effect during cell preparation and/or culture. 2 Prostaglandin E 2 inhibited [ 3 H]‐thymidine incorporation induced by suboptimal phytohaemagglutinin concentrations and had a more potent effect on indomethacin‐treated cells than on untreated cells. 3 Prostaglandin I 2 also exhibited an inhibitory effect and, under defined conditions, was more potent than prostaglandin E 2 or than prostacyclin which had been allowed to decay at pH 7.4 and 37°C. 4 These results indicate that, in attempting to define altered lymphocyte reactivity in disease states, the involvement of prostaglandins should be considered both during cell preparation and culture.