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STUDY ON THE POSSIBLE ENTRY OF BACTERIAL ENDOTOXIN AND PROSTAGLANDIN E 2 INTO THE CENTRAL NERVOUS SYSTEM FROM THE BLOOD
Author(s) -
DASCOMBE M.J.,
MILTON A.S.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb13695.x
Subject(s) - cats , metabolite , prostaglandin e , prostaglandin , cerebrospinal fluid , central nervous system , hypothalamus , prostaglandin e2 , preoptic area , endocrinology , chemistry , medicine , in vivo , fissipedia , pharmacology , biology , microbiology and biotechnology
1 A study has been made of the possible entry of 51 Cr‐bacterial endotoxin and [5,6,8,11,12,14,15(n)‐ 3 H]‐prostaglandin E 2 ([ 3 H]‐PGE 2 ) into the CNS of the anaesthetized cat. 2 No radioactivity was detected in perfusates of the preoptic‐anterior hypothalamus or in the cerebrospinal fluid (c.s.f.) in vivo , or in brain tissue post mortem following intracarotid infusion of 51 Cr‐bacterial endotoxin. 3 Intracarotid administration of [ 3 H]‐PGE 2 resulted in the entry of radioactivity into the CNS of endotoxin pretreated cats. Chromatographic analysis indicated the radioactivity in c.s.f. to be associated with PGE 2 and a metabolite similar to 13, 14‐dihydro‐15‐keto PGE 2 . 4 Intracarotid administration of 13, 14‐dihydro‐15‐keto [5,6,8,11,12,14(n)‐ 3 H]‐PGE 2 resulted in the presence of the compound in the CNS of the anaesthetized cat after pretreatment with bacterial endotoxin. 5 It is concluded that PGE 2 and possibly 13,14‐dihydro‐15‐keto PGE 2 but not bacterial endotoxin may enter the CNS from the cerebral circulation to elicit the febrile response to bacterial endotoxin in cats.