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THE RELEASE OF PROSTAGLANDIN E 2 FROM THE SKIN OF THE PLAICE, Pleuronectes platessa L.
Author(s) -
ANDERSON A.A.,
FLETCHER THELMA C.,
SMITH G.M.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb13693.x
Subject(s) - pleuronectes , incubation , prostaglandin , eicosapentaenoic acid , chemistry , arachidonic acid , prostaglandin e2 , fatty acid , polyunsaturated fatty acid , chromatography , biochemistry , endocrinology , fish <actinopterygii> , biology , fishery , enzyme
1 A fungal extract which produces a cutaneous hypersensitivity reaction in the plaice, Pleuronectes platessa L. , was incubated in vitro with the skin of this teleost fish. Samples of incubation media were assayed for smooth muscle stimulating activity. 2 Prostaglandin E 2 was identified by bioassay, thin‐layer chromatography, ultraviolet absorption spectroscopy and gas chromatography‐mass spectrometry. Release from challenged skin was maximum after 60 min incubation. 3 Analysis of the fatty acid composition of plaice skin showed that although arachidonic acid was present (3% of total fatty acids), the precursor of prostaglandin E 3 , eicosapentaenoic acid contributed 9% of total. 4 Indomethacin (50 mg/kg i.p.) did not inhibit the erythema induced by the fungal extract, whilst a dose of 1 mg/kg maximally inhibited prostaglandin release from skin on incubation in vitro . 5 It is concluded that prostaglandins do not have an exclusive role in the mediation of the hypersensitivity reaction.