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A STUDY OF THE MUSCARINIC RECEPTOR BY GEL ELECTROPHORESIS
Author(s) -
BIRDSALL N.J.M.,
BURGEN A.S.V.,
HULME E.C.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb13685.x
Subject(s) - muscarinic acetylcholine receptor , proteolysis , gel electrophoresis , atropine , chemistry , receptor , electrophoresis , guinea pig , sodium , biochemistry , chromatography , microbiology and biotechnology , biology , endocrinology , organic chemistry , enzyme
1 Muscarinic receptors from the brain of rat, guinea‐pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([ 3 H]‐PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis a single radiolabeled peak was seen which was completely suppressed when labelling was carried out in the presence of 10 −6 m atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of ∼80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.