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IMPROVED INSULIN RECEPTOR ASSAY: EFFECTS OF AN ANTIDIABETIC SULPHONYLUREA ON LIVER MEMBRANE INSULIN RECEPTORS FROM OBESE HYPERGLYCAEMIC MICE
Author(s) -
GREENSTEIN B.D.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb13682.x
Subject(s) - endocrinology , medicine , receptor , insulin , incubation , insulin receptor , membrane , chemistry , population , insulin receptor substrate , biology , insulin resistance , biochemistry , environmental health
1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin‐receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co‐operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13°C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF‐26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold.