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INHIBITION OF GUINEA‐PIG LYMPHOCYTE ACTIVATION BY HISTAMINE AND HISTAMINE ANALOGUES
Author(s) -
BEETS J.L.,
DALE M. MAUREEN
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb10839.x
Subject(s) - histamine , guinea pig , lymphocyte , pharmacology , chemistry , lymphocyte activation , immunology , medicine , endocrinology , immune system , t cell
1 The incorporation of [ 3 H]‐thymidine into guinea‐pig lymphocytes stimulated by a plant lectin (concanavalin A), soluble antigen (tuberculin (P.P.D.)) and syngeneic hepatoma cells, was partially inhibited (50%) by histamine in vitro . 2 The effect of histamine on both mitogen and antigen dose‐response curves suggests a non‐competitive, probably physiological antagonism. 3 The inhibitory dose range of histamine lay between 10 n m and 30 μ m with an ID 50 of approximately 400 n m . 4 The potency order for histamine analogues for the inhibition of lymphocyte activation was histamine ≥ 4‐methylhistamine > 2‐methylhistamine > 3‐methylhistamine. This is in accord with the mediation of the response through an H 2 ‐receptor. 5 H 2 ‐receptor antagonists reversed the inhibitory effect of histamine in a dose‐related manner, but both metiamide and burimamide, in high concentrations, augmented lymphocyte activation in their own right. This precluded the determination of affinity constants and made it impossible to state with certainty that the inhibition of lymphocyte activation by histamine was mediated by an H 2 ‐receptor.