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THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION AND MOBILISATION OF ARACHIDONIC ACID IN FAT CELL GHOSTS
Author(s) -
LEWIS G.P.,
PIPER PRISCILLA J.,
VIGO CARMEN
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb08693.x
Subject(s) - arachidonic acid , chemistry , distribution (mathematics) , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , mathematical analysis , mathematics , enzyme
1 The prostaglandin synthesizing enzymes were found to be present in fat cell ghosts isolated from rabbit adipose tissue. 2 Prostaglandin E 2 (PGE 2 ) and PGF 2 were synthesized by ghosts after stimulation with adrenocor‐ticotrophic hormone (ACTH). 3 Indomethacin was found to inhibit this synthesis but not the synthesis of lipoxygenase products. 4 When fat cell ghosts were stimulated by ACTH, fatty acid release was observed from both neutral lipids and phospholipids. 5 The arachidonic acid (AA) pool within the ghosts was identified: approximately 90% was present in the phospholipid fraction, 8.5% in the neutral lipids and 1.5% unbound. 6 The glucocorticoids were found to stimulate incorporation of [ 14 C]‐AA into neutral lipids and inhibit its incorporation into phospholipids. 7 When fatty acid release was stimulated with ACTH, the glucocorticoids were found to inhibit the mobilisation of [ 14 C]‐AA from the phospholipids and enhance its release from the neutral lipids. 8 The glucocorticoids inhibit prostaglandin formation in fat cell ghosts.