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ACTION OF PROSTAGLANDIN DEHYDROGENASE INHIBITORS ON PROSTAGLANDIN UPTAKE IN RAT ISOLATED LUNG
Author(s) -
BAKHLE Y.S.
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb07875.x
Subject(s) - prostaglandin , chemistry , prostaglandin e2 , maleimide , pharmacology , prostaglandin e , dehydrogenase , lung , efflux , bioassay , biochemistry , endocrinology , medicine , biology , enzyme , genetics , polymer chemistry
1 The effects of some inhibitors of 15‐hydroxy‐prostaglandin dehydrogenase (PGDH) have been studied on the inactivation of prostaglandin E 2 (PGE 2 ) and 16, 16‐dimethyl PGE 2 in rat isolated lung. 2 Bioassay was used to show that the inactivation of PGE 2 and its methyl analogue, was inhibited by frusemide (10 −3 m ), N‐ethyl maleimide (10 −5 m ) and 5, 5′‐dithio‐bis‐2‐nitro‐benzoic acid (10 −2 m ), but not by caffeine (2.5 × 10 −3 m ). 3 The efflux of radioactivity from lung following injection of [ 14 C]‐PGE 2 was faster in lungs treated with frusemide, N‐ethyl maleimide or bromcresol green, than in untreated lungs. 4 Caffeine (10 −3 , 10 −2 m ) did not change the rate of 14 C‐efflux from lungs following injection of [ 14 C]‐PGE 2 . 5 From these results it is concluded that those PGDH inhibitors that prevented prostaglandin inactivation in isolated lungs did so by inhibiting uptake of prostaglandin rather than by inhibiting PGDH.