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STABILIZATION OF LYSOSOMES IN ANOXIC MYOCARDIUM BY PROPRANOLOL
Author(s) -
WELMAN ELIZABETH
Publication year - 1979
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1979.tb07854.x
Subject(s) - propranolol , lysosome , chemistry , perfusion , microgram , guinea pig , pharmacology , medicine , endocrinology , in vitro , biochemistry , biology , enzyme
1 Isolated hearts of guinea‐pigs were perfused aerobically and anoxically for 60 min. (±)‐Propranolol was added to the perfusion medium in concentrations ranging from 10 ng to 10 μg/ml. 2 Lysosome stability was assessed by measurements of latent acid hydrolase activity in homogenates of left ventricular tissue. 3 In the absence of propranolol, the integrity of the myocardial lysosomes was considerably reduced after 60 min anoxia. Lysosome stability was enhanced by the presence of (±)‐propranolol. The optimal concentration was found to be 0.1 μg/ml. This concentration of the (+)‐isomer alone was less effective. 4 It was concluded that bT‐adrenoceptor blockade was the major mechanism by which lysosome disruption was prevented but that some direct membrane effect of propranolol may also be involved.