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THE RELEASE OF PROSTAGLANDINS AND THROMBOXANES FROM GUINEA‐PIG LUNG BY SLOW REACTING SUBSTANCE OF ANAPHYLAXIS, AND ITS INHIBITION
Author(s) -
ENGINEER DINAZ M.,
MORRIS H.R.,
PIPER PRISCILLA J.,
SIROIS P.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb17291.x
Subject(s) - microgram , chemistry , guinea pig , incubation , prostaglandin , thromboxane , thromboxane b2 , thromboxanes , medicine , pharmacology , endocrinology , biochemistry , enzyme , platelet , biology , arachidonic acid , in vitro
1 Rabbit aorta contracting substance (RCS; consisting mainly of thromboxane A 2 ) and prostaglandin‐like material were released from guinea‐pig isolated perfused lungs by injection of slow reacting substance of anaphylaxis (SRS‐A). 2 SRS‐A was resistant to boiling and proteolytic enzymes and was therefore distinguished from rabbit aorta contracting substance releasing factor (RCS‐RF). 3 The release of RCS and prostaglandin‐like material by SRS‐A was anatagonized by indomethacin (1 μg/ml), betamethasone and dexamethasone (4 to 50 μg/ml). 4 Imidazole (200 μg/ml) inhibited the formation of thromboxane A 2 but not that of prostaglandins. 5 The activity of SRS‐A on guinea‐pig ileum and its ability to release RCS and prostaglandins were destroyed by incubation with arylsulphatase (0.83 μg to 1 mg/ml) and with lipoxidase (16.5 to 50 μg/ml): SRS‐A lost activity on incubation with bovine serum albumin (9 μg/ml) due to protein binding.