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RAT ISOLATED PHRENIC NERVE‐DIAPHRAGM PREPARATION FOR PHARMACOLOGICAL STUDY OF MUSCLE SPINDLE AFFERENT ACTIVITY: EFFECT OF OXOTREMORINE
Author(s) -
GANGULY D.K.,
NATH D.N.,
ROSS HG.,
VEDASIROMONI J.R.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb08639.x
Subject(s) - oxotremorine , muscle spindle , phrenic nerve , acetylcholine , physostigmine , chemistry , atropine , neuromuscular junction , cholinergic , anatomy , curare , afferent , medicine , neuroscience , biology , muscarinic acetylcholine receptor , respiratory system , receptor , biochemistry
1 Muscle spindle afferent discharges exhibiting an approximately linear length‐frequency relation could be recorded from the phrenic nerve in the isolated phrenic nerve‐diaphragm preparation of the rat. 2 Muscle spindle afferent discharges could be identified by their characteristic ‘spindle pause’ during muscle contraction and by their response to succinylcholine. 3 Cholinergic influence on spontaneous and stretch‐induced afferent discharges was indicated by the augmentation produced by physostigmine and acetylcholine. (+)‐Tubocurarine, but not atropine, prevented this augmentation indicating the presence of curariform cholinoceptors in muscle spindles. 4 Acetylcholine did not appear to be involved in the genesis of spindle afferent discharges as incubation with hemicholinium‐3 and (+)‐tubocurarine failed to affect the rate of spontaneous and stretch‐induced spindle discharges. 5 Oxotremorine markedly increased the rate of spontaneous and stretch‐induced spindle afferent discharges and this effect was prevented in the presence of hemicholinium‐3 and (+)‐tubocurarine. 6 These results with oxotremorine are of interest in connection with the observation that muscle spindle afferents are hyperactive in Parkinsonian patients.