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PHARMACOLOGY OF THE ADRENOCEPTORS AND CHOLINOCEPTORS OF THE BC3HI NONFUSING MUSCLE CELL LINE
Author(s) -
MAUGER JEANPIERRE,
MOURA ANNEMARIE,
WORCEL MANUEL
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb08637.x
Subject(s) - pharmacology , muscle contraction , cats , adrenergic receptor , medicine , endocrinology , neuroscience , chemistry , biology , receptor
1 We have studied the action of different transmitters on the transmembrane 42 K or 86 Rb efflux from tissue cultures of the BC 3 H1 muscle cell line. 2 The effect of catecholamines and carbachol (CCh) on the isotope efflux rate was measured by addition of the drugs at different times during the washout. 3 Noradrenaline (NA), phenylephrine (Phe), isoprenaline (Iso) and CCh increased 42 K and 86 Rb efflux rate in a dose‐dependent manner. 4 The action of NA seems to be due exclusively to the stimulation of α‐receptors, since its effect was blocked by phentolamine but not by propranolol. The effects of Iso on the 86 Rb efflux were inhibited by propranolol. The β‐receptors in the BC 3 H1 cells seem to be of the β 2 ‐type since they are stimulated by Iso and insensitive to NA. 5 The effect of CCh was blocked by (‐h)‐tubocurarine but not by atropine. This result confirms the presence of nicotinic receptors in BC 3 H1 cells.