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INITIAL INVESTIGATIONS INTO ADRENALINE ACCUMULATION AND ADRENERGIC RESPONSIVENESS IN CULTURED NEONATAL RAT HEART CELLS
Author(s) -
WALKER M.J.A.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb08444.x
Subject(s) - incubation , adrenergic receptor , endocrinology , myocyte , medicine , adrenergic , epinephrine , heart cells , cell culture , chemistry , alpha (finance) , biology , receptor , biochemistry , genetics , construct validity , nursing , patient satisfaction
1 Accumulation of tritiated adrenaline (and/or its products) has been studied by a variety of techniques in viable, attached and free‐floating myoblasts cultured from neonatal rat heart cells. 2 Accumulation increased with the number of cells used, the culture age of cells, incubation time and the concentration of adrenaline in the incubation mixture. 3 Accumulation‐concentration data were not linearly correlated with dose‐response data. 4 Accumulation did not correlate well with β‐adrenoceptor responsiveness in a number of situations of varying responsiveness. 5 Accumulation was not blocked by a variety of uptake blocking agents or α‐ and β‐adrenoceptor antagonists. 6 A much smaller accumulation process was observed in fibroblasts which respond to adrenaline with reversible morphological changes.