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THE METABOLISM OF PROSTAGLANDINS BY THE GUINEA‐PIG UTERUS WITH PARTICULAR REFERENCE TO CORPUS LUTEAL MAINTENANCE IN EARLY PREGNANCY 1
Author(s) -
WALKER FELICITY M. MAULE,
POYSER N.L.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb08443.x
Subject(s) - uterus , conceptus , luteal phase , guinea pig , endocrinology , medicine , nad+ kinase , metabolism , prostaglandin , biology , prostaglandin f2alpha , arachidonic acid , andrology , ovulation , chemistry , pregnancy , biochemistry , gestation , follicular phase , hormone , enzyme , genetics
1 Homogenized lung tissue from day 15, pregnant and non‐pregnant guinea‐pig metabolized exogenous prostaglandin E 2 (PGE 2 ) and prostaglandin F 2α (PGF 2α ) almost completely when incubated in Tyrode solution containing β‐nicotinamide adenine dinucleotide (NAD + ). 2 Metabolism of PGE 2 and PGF 2α by homogenates of day 15, pregnant or non‐pregnant, guinea‐pig uteri was relatively low (8 to 20%) when incubated in Tyrode solution containing NAD + . 3 Day 15, guinea‐pig conceptuses (placentae and embryos), homogenized and incubated in Tyrode solution containing NAD + , metabolized prostaglandins to a slightly greater extent (14 to 22%) than day 15, uterine tissue. 4 Addition of exogenous arachidonic acid to day 12 or day 15, pregnant, guinea‐pig uterine homogenates in Tyrode solution did not increase the yield of PGF 2α following incubation. 5 It is concluded that neither an increase in metabolism of PGF 2α by the uterus and conceptus nor lack of precursor is responsible for the lower secretion of PGF 2α from the day 15, pregnant, guiena‐pig uterus when compared to the day 15, non‐pregnant uterus.