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PRESYNAPTIC ACTIONS OF β‐AMINOBUTYRIC ACID AND SOME ANTAGONISTS IN A SLICE PREPARATION OF CUNEATE NUCLEUS
Author(s) -
SIMMONDS M.A.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb07803.x
Subject(s) - cuneate nucleus , aminobutyric acid , neuroscience , chemistry , nucleus , biochemistry , biology , receptor
1 A slice preparation of the rat cuneate nucleus is described which is suitable for electrophysiological studies on the presynaptic action of drugs. 2 Superfusion of a slice with γ‐aminobutyric acid (GABA) depolarized the afferent nerves in a concentration‐related manner. The responses were CI “‐dependent. Depolarizations to high concentrations of GABA often faded. Glycine and l ‐glutamate had little effect. 3 (‐h)‐Bicuculline antagonized GABA in an apparently competitive manner (pA 2 = 5.35) at low response levels. Strychnine was 10 times less potent. Responses to high concentrations of GABA were sometimes potentiated by (+)‐biculline and strychnine. 4 Bemegride and leptazol both antagonized GABA, but with low potency and in a manner which was clearly not competitive.