Premium
BLOCKADE OF DESENSITIZATION OF NICOTINIC RECEPTORS OF THE CAT ADRENAL MEDULLA BY CONCANAVALIN A
Author(s) -
KIRPEKAR S.M.,
PRAT J.C.
Publication year - 1978
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1978.tb07760.x
Subject(s) - endocrinology , adrenal medulla , medicine , desensitization (medicine) , nicotinic agonist , adrenal gland , catecholamine , receptor , chemistry , stimulation , acetylcholine , muscarinic acetylcholine receptor , biology
1 The possibility of concanavalin A (Con A) blocking the development of desensitization of nicotinic receptors of the cat adrenal gland has been investigated. 2 During perfusion of the adrenal gland with Krebs‐bicarbonate solution containing acetylcholine (ACh), the rate of catecholamine (CA) secretion was very high in the first 2 min; thereafter, as perfusion with ACh was continued the output fell, to reach about 20% of the initial value in 10 minutes. When the adrenal gland was pretreated with Con A, the subsequent desensitization of release during continued infusion of ACh was prevented. 3 When the adrenal gland was perfused with high K + solution, there was always a large initial secretion of CA, and as perfusion with high K + continued the output fell, to reach about 15% of the initial rate in 10 minutes. Con A did not affect the rate of CA secretion induced by high K + . 4 It is tentatively suggested that Con A blocks the desensitization of CA secretion evoked by ACh by interaction with the glycoprotein moiety of the nicotinic receptor of adrenal chromaffin cells.