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THE EFFECT OF CLOPROSTENOL ON HUMAN LUTEAL STEROID AND PROSTAGLANDIN SECRETION in vitro
Author(s) -
McDOUGALL A.N.,
WALKER FELICITY M. MAULE,
WATSON J.
Publication year - 1977
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1977.tb07518.x
Subject(s) - luteal phase , endocrinology , medicine , stimulation , prostaglandin , secretion , prostaglandin e2 , biology , chemistry , hormone
1 Human luteal tissue slices from days 18, 21 and 25 of the menstrual cycle were superfused in vitro with Medium 199 alone or containing cloprostenol (1 μg/ml). Concentrations of progesterone, oestradiol‐17β and prostaglandins F 2α and E 2 were determined in the superfusate samples. 2 Secretion of steroids and prostaglandins was maintained at an approximately constant level throughout the experiments (21 h in one case) when the tissue was perfused with M199 alone. 3 Superfusion with cloprostenol (1 μg/ml) resulted in an initial depression of progesterone and oestradiol‐17β but this was not maintained, levels returning to control values or showing an increase, while superfusion with cloprostenol continued. Cloprostenol is not therefore considered to be luteolytic at this dose and under these conditions for human luteal tissue in vitro. 4 Superfusion with cloprostenol (1 μg/ml) also resulted in a large stimulation of secretion of endogenous prostaglandin F 2α following a short lag phase. This stimulation was possibly due to the initial depression of progesterone secretion. A short‐lived stimulation of prostaglandin E 2 secretion was also observed. 5 The significance of the increase in prostaglandin E 2 secretion and the interrelationships between the various changes observed with cloprostenol are difficult to interpret.