Premium
EFFECT OF p ‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A 2 , ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro
Author(s) -
MITCHELL SHEILA,
POYSER N.L.,
WILSON N.H.
Publication year - 1977
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1977.tb06983.x
Subject(s) - arachidonic acid , prostaglandin f2alpha , prostaglandins f , guinea pig , uterus , endocrinology , prostaglandin , medicine , phospholipase a2 , chemistry , phospholipase a , biochemistry , biology , enzyme
1 The synthesis of prostaglandins F 2α and E 2 by guinea‐pig uterine homogenates was inhibited by p ‐bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A 2 . 2 Metabolism of prostaglandin F 2α by uterine homogenates was undetectable; this was not affected by PBPAB. 3 There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7 and 15 of the oestrous cycle. Small amounts of dihomo‐γ‐linolenic acid were detected in the homogenates. 4 The release of arachidonic acid from uterine homogenates was greatly inhibited by PBPAB. 5 Addition of exogenous arachidonic acid to uterine homogenates did not overcome the inhibition of uterine prostaglandin F 2α synthesis produced by PBPAB. 6 It is concluded that PBPAB inhibits both the release of arachidonic acid from the guinea‐pig uterus and its subsequent conversion into prostaglandins.