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A RAPID METHOD FOR THE ESTIMATION OF PROSTAGLANDIN 15‐HYDROXYDEHYDROGENASE ACTIVITY AND ITS APPLICATION TO PHARMACOLOGY
Author(s) -
BLACKWELL G.J.,
FLOWER R.J.
Publication year - 1976
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1976.tb10389.x
Subject(s) - prostaglandin , enzyme , chemistry , pharmacology , biochemistry , biology
1 A novel double‐isotope assay for prostaglandin 15‐hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell‐free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.