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INHIBITION OF PERISTALSIS IN GUINEA‐PIG ISOLATED ILEUM AND COLON BY DRUGS THAT BLOCK PROSTAGLANDIN SYNTHESIS
Author(s) -
BENNETT A.,
ELEY K.G.,
STOCKLEY HELEN L.
Publication year - 1976
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1976.tb07671.x
Subject(s) - peristalsis , ileum , guinea pig , prostaglandin , pharmacology , chemistry , block (permutation group theory) , medicine , mathematics , geometry
1 Methods of analysing peristaltic activity have been evaluated by the use of recordings of longitudinal and circular muscle activity and of propulsion in whole segments of guinea‐pig ileum and colon. 2 Some prostaglandin synthesis inhibitors, and antagonists of prostaglandin action were tested for their suitability for studying the role of prostaglandins in peristalsis. Aspirin was suitable; at 10–200 μg/ml it had little effect on responses of longitudinal muscle strips of the guinea‐pig ileum to acetylcholine (ACh), histamine, nicotine or prostaglandin E 2 . Indomethacin (1–4 μg/ml) reduced responses to nicotine and prostaglandin E 2 . The prostaglandin antagonists polyphloretin phosphate and SC‐19220 reduced contractions of ileal longitudinal muscle caused by nerve excitation with either nicotine or transmural stimulation. 3 Aspirin (20–100 μg/ml) or indomethacin (1–4 μg/ml) applied serosally greatly inhibited all aspects of peristalsis in guinea‐pig ileum and colon. Inhibition of peristalsis of the ileum by aspirin was antagonized by prostaglandin E 2 and that by indomethacin was removed by prostaglandin F 2α or ACh. Inhibition of colonic peristalsis by aspirin was antagonized by prostaglandin E 2 but rarely by ACh, and that by indomethacin by prostaglandin E 1 or E 2 . Mucosal application of aspirin had little effect on either ileum or colon but indomethacin caused some inhibition. 4 These results support the supposition that prostaglandins contribute to peristaltic activity.