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INDOMETHACIN‐INDUCED ALTERATIONS IN CORTICOSTEROID AND PROSTAGLANDIN RELEASE BY ISOLATED ADRENOCORTICAL CELLS OF THE CAT
Author(s) -
LAYCHOCK SUZANNE G.,
RUBIN R.P.
Publication year - 1976
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1976.tb07477.x
Subject(s) - prostaglandin , medicine , endocrinology , steroid , prostaglandin e2 , chemistry , basal (medicine) , prostaglandin e , steroid hormone , liberation , biology , in vitro , biochemistry , hormone , insulin
1 The effects of purported prostaglandin synthesis inhibitors on steroid and prostaglandin (E and F) release from trypsin‐dispersed cat adrenocortical cells were investigated. 2 Low indomethacin concentrations potentiated adrenocorticotrophin (ACTH)‐evoked prostaglandin and steroid release, whereas higher concentrations depressed both responses to ACTH. The steroidogenic response to exogenous prostaglandin E 2 was not markedly altered over a wide range of indomethacin concentrations. 3 Indomethacin enhanced basal steroid release but did not enhance basal prostaglandin E or F release. 4 5, 8, 11, 14‐Eicosatetraynoic acid (ETA) elicited a concentration‐dependent inhibition of ACTH‐induced steroid release, but had little effect on prostaglandin E 2 ‐induced steroid release. A high concentration of ETA inhibited prostaglandin E and F release. 5 These data are discussed in relation to the concept that prostaglandins provide a critical link in ACTH‐induced corticosteroidogenesis.