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THE UPTAKE OF TRITIATED Δ 1 ‐TETRAHYDROCANNABINOL BY THE ISOLATED VAS DEFERENS OF THE RAT
Author(s) -
EGAN SANDRA M.,
GRAHAM J.D.P.,
LEWIS M.J.
Publication year - 1976
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1976.tb07451.x
Subject(s) - vas deferens , chemistry , tetrahydrocannabinol , sorbitol , chromatography , δ9 tetrahydrocannabinol , delta 9 tetrahydrocannabinol , radiochemistry , endocrinology , cannabinoid , biochemistry , biology , receptor
1 Weighed stripped vasa deferentia were incubated in Holman's solution containing (a) [ 14 C]‐sorbitol 0.014 mM, (b) [ 3 H]‐noradrenaline ([ 3 H]‐NA) 12.31 nM, (c) [ 3 H]‐tetrahydrocannabinol ([ 3 H]‐Δ 1 ‐THC) 1 μg/ml for 5, 10, 20 and 30 minutes. 2 Tissues were washed, dissolved in Protosol, counted by standard scintillation counting technique and ‘drug space’ expressed as ct min −1 mg −1 tissue/ct min −1 μl −1 bathing fluid. 3 Vasa incubated for 30 min with [ 14 C]‐sorbitol were washed for varying lengths of time; 82% clearance had taken place after 2 washes of 5 minutes. 4 The uptake of [ 3 H]‐NA was inhibited by the presence of desmethylimipramine (DMI) 10 nM in the bath or by pretreatment of rats with 6‐hydroxydopamine (6‐OHDA). 5 The uptake of [ 3 H]‐Δ 1 ‐THC was not inhibited by the presence of DMI. It was reduced but not abolished by 6‐OHDA pretreatment.