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BIOASSAY OF HISTAMINE IN THE PRESENCE OF PROSTAGLANDINS
Author(s) -
GRYGLEWSKI R.J.,
KORBUT R.
Publication year - 1976
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1976.tb06956.x
Subject(s) - bioassay , histamine , amberlite , chromatography , chemistry , bicarbonate , pharmacology , biology , organic chemistry , genetics , adsorption
1 An Amberlite XAD‐2 column in a heated (37°C) jacket was incorporated in between two banks of bioassay organs superfused with Krebs bicarbonate solution in cascade. The column removed prostaglandins E 1 , E 2 and F 2α and also rabbit aorta contracting substance (RCS) and possibly slow reacting substance of anaphylaxis (SRS‐A). 2 The column gave free passage to histamine in concentrations up to 3000 ng/ml. The method described improves the accuracy of histamine bioassay in the presence of prostaglandins.