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EFFECTS OF OESTRADIOL AND PROGESTERONE ON THE in vitro PRODUCTION OF PROSTAGLANDIN F 2α BY THE GUINEA‐PIG UTERUS
Author(s) -
NAYLOR B.,
POYSER N.L.
Publication year - 1975
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1975.tb07632.x
Subject(s) - prostaglandin f2alpha , medicine , endocrinology , uterus , ovariectomized rat , guinea pig , prostaglandin , chemistry , hormone , estrous cycle , prostaglandins f , biology
1 The effects of oestradiol and progesterone on the production of prostaglandin F 2α by the guinea‐pig uterus in vitro have been studied. 2 Ovariectomized guinea‐pigs were treated with oestradiol benzoate, progesterone, or a combination of these hormones. The uteri were homogenized and incubated and the prostaglandin F 2α produced was measured by radioimmunoassay or combined gas chromatography‐mass spectrometry (g.c.‐m.s.). Oestradiol treatment or progesterone followed by oestradiol caused a significant increase in prostaglandin F 2α synthesis compared with control values, whereas progesterone treatment caused no significant increase. 3 The uteri from guinea‐pigs on day 7 of the oestrous cycle were split into four parts, and each part homogenized and incubated either alone or in the presence of oestradiol‐17β, progesterone, or a combination of these hormones. Prostaglandin F 2α was measured by g.c.‐m.s. Oestradiol was found to increase prostaglandin F 2α synthesis by the uterus whereas progesterone had no effect. However, progesterone did inhibit the response to oestradiol. 4 Oestradiol treatment of the guinea‐pig uterus causes an increase in capacity to produce prostaglandin F 2α in vitro . The role of progesterone is difficult to interpret.