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INTERACTION OF MONO‐ AND DIVALENT METALLIC CATIONS AND OF INDOMETHACIN ON THE MEMBRANE POTENTIAL OF VASCULAR ENDOTHELIAL CELLS in vitro
Author(s) -
NORTHOVER B.J.
Publication year - 1975
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1975.tb07617.x
Subject(s) - depolarization , chemistry , divalent , calcium , potassium , ion , inorganic chemistry , ferrous , hydrogen peroxide , membrane potential , metal , biophysics , biochemistry , organic chemistry , biology
1 Endothelial cells depolarized progressively when heated for 5 h at 45° C in the presence of calcium (1 mM), cupric (0.08 mM) or ferrous (0.04 mM) ions. In the absence of these ions, heating caused only slight depolarization. Higher concentrations of these ions caused depolarization even at normal body temperature (37° C). 2 Cuprous and potassium ions, although producing depolarization at 37° C, failed to augment the depolarization due to heating to 45° C. 3 Hydrogen peroxide caused depolarization which was potentiated by the presence of calcium, cupric or ferrous ions, but not by the presence of cuprous or potassium ions. 4 Indomethacin (0.25 mM) reduced the depolarization caused by calcium, cupric or ferrous ions at 37° C and also reduced the potentiation of heat‐induced and hydrogen peroxide‐induced depolarization which these divalent metallic cations produced. However, indomethacin failed to modify the depolarization caused by cuprous or potassium ions.