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THE DEVELOPMENT OF ANTISERA TO PROSTAGLANDINS B 2 AND F 2α AND THEIR ANALYSIS USING SOLID‐PHASE AND DOUBLE ANTIBODY RADIOIMMUNOASSAY METHODS
Author(s) -
DIGHE K.K.,
EMSLIE H.A.,
HENDERSON L.K.,
SIMON L.,
RUTHERFORD F.
Publication year - 1975
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1975.tb07425.x
Subject(s) - radioimmunoassay , antiserum , bovine serum albumin , chemistry , chromatography , prostaglandin , conjugate , prostaglandin e , antibody , biochemistry , biology , immunology , mathematical analysis , mathematics
1 A method for the production of highly substituted prostaglandin‐bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B 2 and F 2α were raised in rabbits immunized with prostaglandin‐bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a ‘solid‐phase’ system. 4 Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.