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A CHEMOTACTIC ROLE FOR PROSTAGLANDINS RELEASED FROM POLYMORPHONUCLEAR LEUCOCYTES DURING PHAGOCYTOSIS
Author(s) -
HIGGS G.A.,
McCALL ELAINE,
YOULTEN L.J.F.
Publication year - 1975
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1975.tb07392.x
Subject(s) - phagocytosis , chemotaxis , prostaglandin , prostaglandin e , prostaglandin e2 , bioassay , arachidonic acid , prostaglandins f , chemistry , inflammation , granulocyte , prostaglandin e1 , bacteria , endocrinology , biology , microbiology and biotechnology , medicine , biochemistry , immunology , enzyme , receptor , genetics
1 Prostaglandin E 1 is chemotactic at concentrations down to 10 ng/ml for rabbit polymorphonuclear (PMN) leucocytes. Prostaglandins E 2 and F 2α have little or no chemotactic effect at concentrations up to 10 μg/ml. 2 Washed PMN leucocytes produce a chemotactic agent during phagocytosis, but not in the presence of indomethacin (28 μM). 3 Phagocytosing PMN leucocytes produce up to ten times as much prostaglandin as do resting cells. Some of this is prostaglandin E 1 as judged by thin layer chromatography and differential bioassay. This prostaglandin production by PMN leucocytes is abolished by indomethacin (28 μM). 4 Ultrasonicated suspensions of PMN leucocytes produce prostaglandin from arachidonic acid. This synthesis is inhibited by indomethacin. 5 Homogenates of PMN leucocytes which have been pre‐incubated with bacteria for 30 min show more prostaglandin synthetase activity than homogenates from PMN leucocytes which have not been exposed to bacteria. 6 It is concluded that in some forms of inflammation, prostaglandin E 1 may play a controlling role in cellular migration. 7 PMN leucocytes may contribute to the generation of prostaglandins found in some inflammatory lesions.