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EFFECTS OF ADRENALINE, NORADRENALINE, ISOPRENALINE AND SALBUTAMOL ON THE PRODUCTION AND RELEASE OF RENIN BY ISOLATED RENAL CORTICAL CELLS OF THE CAT
Author(s) -
JOHNS E.J.,
RICHARDS H.K.,
SINGER BERTHA
Publication year - 1975
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1975.tb07331.x
Subject(s) - isoprenaline , salbutamol , renin–angiotensin system , medicine , endocrinology , sympathomimetics , chemistry , pharmacology , stimulation , blood pressure , asthma
1 Isolated renal cortical cells of the cat have been demonstrated to produce renin on incubation in vitro. After 2 h of incubation, without added agonist, the total amount of renin in the flask increased by a mean of 27.2%. The increase in renin content of the incubation flask was found to be present in the medium. 2 Noradrenaline (1.18 × 10 −4 M) and adrenaline (1.09 ×; 10‐ 4 M) added to the incubation medium stimulated renin production by 45 and 34% respectively, compared with the incubated controls. Most of the increase in renin production was present in the incubation medium. 3 Isoprenaline did not stimulate renin production. However, when added to the incubation medium at a concentration of 0.72 × 10‐ 4 M there was a significant decrease in the cellular content and a significant increase in the medium content of renin. This increase was at least as great as that observed with adrenaline and noradrenaline. 4 Salbutamol had an effect similar to isoprenaline, i.e. it induced the release of renin into the medium without affecting production. In this respect it was about a third as potent as isoprenaline.