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THE EFFECT OF Δ 1 ‐TETRAHYDROCANNABINOL ON THE UPTAKE OF [ 3 H]‐(—)‐NORADRENALINE BY THE ISOLATED PERFUSED HEART OF THE RAT
Author(s) -
GRAHAM J.D.P.,
LEWIS M.J.,
LI D.M.F.
Publication year - 1974
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1974.tb10686.x
Subject(s) - chemistry , tetrahydrocannabinol , δ9 tetrahydrocannabinol , pharmacology , cannabinoid , biochemistry , receptor , biology
Forty‐eight isolated rat hearts were perfused with Krebs solution and with [ 3 H]‐(–)‐noradrenaline ([ 3 H]‐NA) containing 4.5 nCi/ml in 50 ng/ml, as described by Iversen (1963). To groups of six, cocaine was added to [ 3 H]‐NA in concentrations of 400 N m , 2 and 10 μ m . To the rest Tween 80, 25 μg/ml was added to [ 3 H]‐NA and in three groups Δ 1 ‐THC additionally in concentrations of 560 N m and 2.8 or 14 μ m . Cocaine caused a linear concentration‐related inhibition of uptake; Tween caused a 38.7% inhibition; THC caused additionally a linear concentration‐related inhibition.