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THE EFFECT OF Δ 1 ‐TETRAHYDROCANNABINOL ON THE RELEASE OF [ 3 H]‐(‐)‐NORADRENALINE FROM THE ISOLATED VAS DEFERENS OF THE RAT
Author(s) -
GRAHAM J.D.P.,
LEWIS M.J.,
LI D.M.F.
Publication year - 1974
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1974.tb09705.x
Subject(s) - vas deferens , stimulation , tritium , chemistry , delta 9 tetrahydrocannabinol , tetrahydrocannabinol , sorbitol , endocrinology , medicine , pharmacology , biology , biochemistry , cannabinoid , receptor , physics , nuclear physics
1 The amount of tritium released upon transmural stimulation of the isolated vas of the rat incubated with [ 3 H]‐Δ 1 ‐tetrahydrocannabinol (THC) (2.8 μM) was significantly greater than the non‐stimulated overflow. 2 There was no difference between non‐stimulated overflow and the effect of transmural stimulation of vasa equilibrated with [ 14 C]‐sorbitol (6.5 mg/ml). 3 The difference between non‐stimulated and stimulated efflux from vasa equilibrated with [ 3 H]‐Δ 1 ‐THC was abolished in rats pretreated for 24 h with 6‐hydroxydopamine (250 mg/kg). 4 The amount of tritium released upon transmural stimulation of the vas incubated with [ 3 H]‐noradrenaline (6 μ m ) was significantly greater than the non‐stimulated overflow. 5 Δ 1 ‐THC (560 N m and 14 μ m ) caused a significant dose‐dependent reduction of both non‐stimulated and stimulated tritium efflux, especially the latter.