z-logo
Premium
STUDIES ON THE METABOLISM OF CATECHOLAMINES IN THE CENTRAL NERVOUS SYSTEM OF THE MOUSE
Author(s) -
CEASAR P.M.,
HAGUE P.,
SHARMAN D.F.,
WERDINIUS B.
Publication year - 1974
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1974.tb09646.x
Subject(s) - dopamine , medicine , endocrinology , reserpine , chemistry , ascorbic acid , catechol o methyl transferase , metabolism , catecholamine , striatum , monoamine oxidase , dopaminergic , normetanephrine , tyrosine hydroxylase , catechol , metabolite , tyrosine , norepinephrine , enzyme , biochemistry , biology , allele , food science , gene
1 The distribution of the metabolites of noradrenaline, 1‐(3,4‐dihydroxyphenyl)ethane‐1,2‐diol (DOPEG) and 1‐(4‐hydroxy‐3‐methoxyphenyl)ethane‐1,2‐diol (MOPEG), in the brain of the mouse has been investigated. 2 The rate of disappearance of the metabolites after inhibition of the enzyme monoamine oxidase has been used to estimate their turnover rates in the mouse hypothalamus. It was concluded that the turnover of DOPEG was much faster than that of MOPEG. 3 When mice were treated with reserpine dissolved in 5% ascorbic acid solution there was an increase in the hypothalamic concentration of both MOPEG and DOPEG. However, similar increases in the concentrations of the two metabolites were seen when the animals were treated with 5% ascorbic acid solution alone. 4 The administration of tropolone, an inhibitor of the enzyme catechol‐ O ‐methyl transferase, resulted in an increase in the concentration of DOPEG. 5 Mice, exposed to a temperature of −15° C showed increased hypothalamic concentrations of both DOPEG and MOPEG. 6 The rates of formation in the mouse striatum of 3,4‐dihydroxyphenylacetic acid (DOPAC) and 4‐hydroxy‐3‐methoxyphenylacetic acid (HVA), acidic metabolites of dopamine, were compared with the turnover rate of dopamine, estimated from the rate at which this catecholamine disappears after treatment with α‐methyl‐ p ‐tyrosine. It was concluded that the estimate of dopamine turnover obtained by this method is likely to be too large because of the compensatory feedback mechanism which is thought to play a role in the metabolism of dopamine in the brain.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here