Premium
THE EFFECT OF INDOMETHACIN ON THE RELEASE OF PROSTAGLANDIN E 2 AND ACETYLCHOLINE FROM GUINEA‐PIG ISOLATED ILEUM AT REST AND DURING FIELD STIMULATION
Author(s) -
BOTTING J.H.,
SALZMANN R.
Publication year - 1974
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1974.tb09598.x
Subject(s) - acetylcholine , ileum , stimulation , guinea pig , rest (music) , chemistry , endocrinology , prostaglandin , prostaglandin e , medicine , pharmacology
1 Guinea‐pig ileum suspended in Krebs solution showed a continuous increase of tone which was lost by changing the bath fluid. Prostaglandin E 2 was released from the ileum during incubation, and its concentration in the bath fluid appeared to correlate with the increase in tone. 2 Supramaximal field stimulation (10 Hz) resulted in increased release of prostaglandin E 2 from the ileum. At lower rates of stimulation, the increase in the release of E 2 compared with the resting output was not significant. 3 Indomethacin (1 and 10 μg/ml) produced a significant, dose‐related reduction of the amount of prostaglandin E 2 measured in the bath fluid at rest and with field stimulation. Indomethacin inhibited the contraction of the ileum during incubation in Krebs solution. 4 Indomethacin (10 and 20 μg/ml) had no significant effect on the release of acetylcholine during field stimulation, but reduced the resting output of acetylcholine from guinea‐pig ileum in some experiments. 5 The results are discussed in the context of the role ascribed to prostaglandins as physiological modulators in transmitter release. No evidence for a prostaglandin‐mediated negative feedback mechanism on acetylcholine release was obtained.