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The use of the hamster stomach in vitro as an assay preparation for prostaglandins
Author(s) -
UBATUBA F. B.
Publication year - 1973
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1973.tb08542.x
Subject(s) - hamster , histamine , prostaglandin , stomach , prostaglandin e , prostaglandins f , medicine , chemistry , in vitro , endocrinology , biology , biochemistry
Summary1 Hamster stomach strips in vitro are useful test preparations for the assay of prostaglandins E and F in the nanogram concentration range. Three‐minute cycles can be used for long periods without spontaneous contractions or significant base‐line instability. 2 The mean percentage error and the precision index of six 4‐point assays of prostaglandin E 2 were 7·8 and 0·057, respectively. 3 The relative sensitivity to different prostaglandins was E 2 /E 1 ∼1, E 2 /F 1α ∼65 and E 2 /A 1 ∼400. 4 These preparations were relatively insensitive to 5‐hydroxytryptamine (prostaglandin E 2 /5‐HT∼20,000) and histamine (prostaglandin E 2 /histamine∼200). 5 These results suggest that the hamster stomach preparation can be used to differentiate between 5‐hydroxytryptamine and prostaglandins.