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Inhibition by diuretics of cyclic 3′,5′‐AMP‐dependent protein kinase from toad bladder epithelium
Author(s) -
FERGUSON D. R.,
TWITE B. R.
Publication year - 1973
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1973.tb08374.x
Subject(s) - toad , bumetanide , endocrinology , medicine , protein kinase a , chemistry , epithelium , enzyme , hydrochlorothiazide , biology , biochemistry , sodium , cotransporter , blood pressure , genetics , organic chemistry
Summary1 A cAMP‐dependent protein kinase enzyme has been isolated from toad bladder epithelium. 2 This enzyme catalyses the phosphorylation of histones; a reaction stimulated by 0·1 μ m cAMP. 3 Activity of this enzyme in the presence of 01 μ m cAMP was significantly inhibited by 100 μ m mercuderamide, bumetanide and frusemide. A small, though statistically insignificant inhibition was seen with the same concentrations of hydrochlorothiazide and ethacrynic acid. 4 The half maximal inhibition was achieved with mercuderamide, 5 μ m , and with frusemide and bumetanide 600 μ m .